Course Syllabus for English-Taught Majors

NanobiotechnologyCourse Syllabus


Course Code09040009

Course CategoryMajor Elective

MajorsChemistry (Intensive Training Class)


Total Hours36 Hours         Credit2 学分

Lecture Hours36 Hours          Lab Hours0 Hours      Practice Hours0 Hours

InstructorsNan Ma

TextbooksNanobiotechnology: Concepts, Applications and Perspectives, WILEY-VCH Edited by C. M. Niemeyer, C. A. Mirkin


1. X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, G. Sundaresan, A. M. Wu, S. S. Gambhir, S. Weiss,

Science 2005, 307, 538.

2. I. L. Medintz, H. T. Uyeda, E. R. Goldman, H. Mattoussi, Nat. Mater. 2005, 4, 435.

3. Mirkin, C. A.; Letsinger, R. L.; Mucic, R. C.; Storhoff, J. J. Nature 1996, 382, 607609.

4. N. L. Rosi, D. A. Giljohann, C. S. Thaxton, A. K. Lytton-Jean, M. S. Han, C. A. Mirkin, Science 2006, 312, 1027.

5. A. V. Pinheiro, D. Han, W. M. Shih, H. Yan Nat. Nanotechnol. 2011, 6, 763.



Teaching Aim

   This course will provide a general introduction to the newly emerging field of nanobiotechnology. This is a highly interdisciplinary field covering nanotechnology, chemistry, biology, and medicine. The rapid development of this field has lead to a variety of nano architectures that could be applied to biosensing, molecular imaging, and therapy, which is going to change our lives significantly. This is an advanced course for fourth-year students who have a solid background of chemistry and biochemistry, and those plan to purse a higher degree in the related field.  


Chapter I  Introduction



第一节  Introduction

一、Definition of nanotechnology and nanobiotechnology   

二、Classification and preparation of nanomaterials

三、Characterization methods

四、Journals and search engine

思考题 Problems

1What is nanobiotechnology?

2What is the difference between TEM, AFM and SEM

3Please name five top journals publishing research in the field of nanobiotechnology


Chapter II  Quantum Dots



第一节  Synthesis and properties

一、Discovery of colloidal quantum dots   

二、Quantum confinement effect

三、Optical properties

四、Synthetic methods

第二节  Types and compositions

一、Binary and ternary quantum dots

二、Type I and type II quantum dots

三、Doped quantum dots

四、Strain tuning

第三节  Biofunctionalization

一、Molecules for solubilization and functionalization

二、Aqueous and one-step synthesis

第四节  QDs for biosensing

一、Fluorescence resonance energy transfer

二、Nucleic acids detection

三、Protease detection

四、Bioluminescence resonance energy transfer

第五节  QDs for bioimaging

一、Molecular recognition ligands

二、Intracellular delivery

三、Design consideration for in vivo imaging

四、Tumor imaging strategies

五、Lymph node imaging

六、Drug delivery and cancer therapy

思考题 Problems

1What is quantum confinement effect and how does it determine the photoluminescence properties of QDs

2Please design a FRET-based QD probe for sensitive detection of specific DNA molecules.

3What is the difference between passive and active tumor targeting


Chapter III  Gold nanoparticles



第一节  Synthesis and properties

一、Discovery of gold nanoparticles

二、Synthesis of gold nanoparticles, nanorods, and nanocages

三、Optical properties of gold nanostructures

第二节  GNPs for biosensing

一、Surface plasma resonance

二、Protease detection

三、Nucleic acids detection

四、Small molecule detection

五、Surface enhanced Raman scattering

第三节  GNPs for drug delivery

一、Surface functionalization

二、Drug encapsulation and release

三、Photothermal therapy

思考题 Problems

1What is surface plasma resonance of gold nanoparticles

2Please design a DNA detection method based on gold nanoparticle aggregation

3Please design a FRET-based gold nanoparticle probe for protease detection


Chapter IV  DNA nanotechnology


第一节  DNA self-assembly

一、DNA chemical structure

二、Secondary structure and assembly building blocks

三、DNA-templated nanoparticle assembly

四、DNA origami

五、Dynamic assembly

第二节  Applications


二、Drug delivery

思考题 Problems

1What are the advantages of DNA molecules for nanoscale assembly

2What is DNA origami


Chapter V  Oral presentation and discussion


Present a comprehensive topic on nanobiotechnology



考核方式 Assessment Methodsattendance (10%); oral presentation (40%); report (50%)





                                执笔人 Made by Nan Ma (马楠)

Date:  2016-11-9